Composite
Part:BBa_K4619010:Design
Designed by: KaMing Li Group: iGEM23_UCAS-China (2023-10-09)
PobR-Threshold Guard Switch
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 462
Illegal AgeI site found at 154
Illegal AgeI site found at 675 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 595
Illegal SapI.rc site found at 1573
Design Notes
Regarding the original digitizer[1], we eliminate the XylS system, which includes the XylS protein and its corresponding promoter. We then combine these two sections and anticipate that the new chimeric digitizer will function properly. We use GFP to test our new chimeric digitizer. If it functions well, we will replace GFP with luxI to improve our bio-sensor.
Source
[1] Calles B , Goni-Moreno A , Lorenzo V D .Digitalizing heterologous gene expression in Gram-negative bacteria with a portable on/off module[J]. 2019.DOI:10.1101/783506.