DNA

Part:BBa_K4664002:Design

Designed by: Yiming Dong   Group: iGEM23_HS-China   (2023-10-09)
Revision as of 16:13, 9 October 2023 by Yiming Dong (Talk | contribs) (→‎Design Notes)

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Padlock-miR-223


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Padlock Padlock-miR-223 is the corresponding padlock chosen for the biomarker miRNA-223. The padlock probe for RCA is engineered with a split ligation module for target miRNA binding and a CRISPR–Cas12a detection module whose complementary sequence activates a Cas12a–crRNA complex. miRNA sequences are extracted from a sample, annealed with the padlock and then added to a reaction tube containing the enzymes, reporter and other reagents.

Source

Sequence synthesis through a CRO company.

References