Composite

Part:BBa_K4800054

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-09)
Revision as of 16:00, 9 October 2023 by Registry (Talk | contribs)

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pTrc99a-davB-davA-GabT

davB-davA-GabT gene encodes for carboxylate reductase, alcohol dehydrogenase and transaminase. These enzymes can realize the metabolic pathway from lysine to glutarate semialdehyde, and the three enzymes form a synthetic module including 5-HV.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5122
    Illegal XbaI site found at 6489
    Illegal PstI site found at 6501
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5122
    Illegal PstI site found at 6501
    Illegal NotI site found at 2808
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5122
    Illegal BglII site found at 3897
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5122
    Illegal XbaI site found at 6489
    Illegal PstI site found at 6501
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5122
    Illegal XbaI site found at 6489
    Illegal PstI site found at 6501
    Illegal NgoMIV site found at 4057
    Illegal AgeI site found at 3464
    Illegal AgeI site found at 3713
    Illegal AgeI site found at 5535
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6685
    Illegal BsaI site found at 7753
    Illegal SapI.rc site found at 767


[edit]
Categories
Parameters
None