Regulatory

Part:BBa_K4673029:Design

Designed by: Chieh Yu Lee   Group: iGEM23_Taipei-KCISLK-V1   (2023-10-02)
Revision as of 15:57, 9 October 2023 by KCLK 23T (Talk | contribs)

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AmpR promoter is for ampicillin resistance


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ampicillin promoter is located between T7 terminator and ampicillin resistance in the pET-22b LK vector, and located between the b-lactamase coding region and f1-origin in the pGEM-T EASY vector.


Source

GSL Biotech LLC. (2023). pET-22b(+) Sequence and Map. SnapGene. https://www.snapgene.com/plasmids/pet_and_duet_vectors_(novagen)/pET-22b(%2B)

In addition to the pET-22b-LK vector, the ampicillin promoter is also inserted into the pGEM-T EASY vector purchased from a company called Promega. https://worldwide.promega.com/products/pcr/pcr-cloning/pgem-t-easy-vector-systems/?catNum=A1360

References

Nakano, R., Nakano, A., Yano, H., Okamoto, R., Ryuichi NakanoDepartment of Microbiology and Infectious Diseases, N. M. U., Akiyo NakanoDepartment of Microbiology and Infectious Diseases, N. M. U., Hisakazu YanoDepartment of Microbiology and Infectious Diseases, N. M. U., & Ryoichi OkamotoDepartment of Microbiology, K. U. S. of M. (2017, August 30). Role of AMPR in the high expression of the plasmid-encoded AMPC β-lactamase CFE-1. mSphere. https://journals.asm.org/doi/10.1128/msphere.00192-17