DNA

Part:BBa_K4673022:Design

Designed by: Chieh Yu Lee   Group: iGEM23_Taipei-KCISLK-V1   (2023-10-02)
Revision as of 15:52, 9 October 2023 by KCLK 23T (Talk | contribs)

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Lac promoter binds RNA polymerase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Lac promoter is located between the lac operon and the lac operator in the pGEM-T EASY vector.


Source

The lac promoter is one of the components of the pGEM-T EASY vector, which our team bought from a company called Promega. https://worldwide.promega.com/products/pcr/pcr-cloning/pgem-t-easy-vector-systems/?catNum=A1360

In addition, the lac promoter is also on the pET-22b-LK. GSL Biotech LLC. (2023). pET-22b(+) Sequence and Map. SnapGene. https://www.snapgene.com/plasmids/pet_and_duet_vectors_(novagen)/pET-22b(%2B)


References

Sudheer Menon, Shanmughavel Piramanayakam, & Gopal Agarwal. (2021). Computational identification of promoter regions in prokaryotes and eukaryotes. EPRA International Journal of Agriculture and Rural Economic Research, 21–28. https://doi.org/10.36713/epra7667