Part:BBa_K4673022
Lac promoter binds RNA polymerase
The first step in transcription is the binding of RNA polymerase to a DNA molecule, and it occurs at particular sites called promoters. A promoter is a segment that is located upstream of DNA near the transcription start site, and it ranges from 20 to 200 base pairs at which numerous molecular interactions take place. This crucial DNA sequence exists in both prokaryotes and eukaryotes, but there are distinct differences between the promoter regions of these two cell types.
In eukaryotes, the core promoter sequence is known as the TATA box, and it can be
bound with the RNA polymerase with the assistance of the binding transcription factors that alter the promoter sequence. However, as prokaryotes lack transcription factors, the recognition of promoter sequence happens through the assistance of the RNA polymerase and a sigma factor.
In our team's project, the lac promoter plays an important role in the IPTG induction as it facilitates the utilization of lactose by cells and controls the transcription of the operon, functioning as the binding site for essential regulatory proteins. Since the lac promoter overlaps the lac operon, the RNA polymerase demonstrates its capability to form a binding interaction with the lac promoter and start transcription as long as the lac operon is not occupied by any substances.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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