Regulatory

Part:BBa_K4673026:Design

Designed by: Chieh Yu Lee   Group: iGEM23_Taipei-KCISLK-V1   (2023-10-02)
Revision as of 15:46, 9 October 2023 by KCLK 23T (Talk | contribs) (→‎References)


LacI is a regulated repressor protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Lacl is located between the Lacl_promoter and ROP on the pEt-22b-LK vector.


Source

GSL Biotech LLC. (2023). pET-22b(+) Sequence and Map. SnapGene. https://www.snapgene.com/plasmids/pet_and_duet_vectors_(novagen)/pET-22b(%2B)

References

Hediger, M. A., Nierlich, D. P., & Zabin, I. (1985, October). DNA sequence of the lactose operon: The laca gene and the transcriptional termination region. Proceedings of the National Academy of Sciences of the United States of America. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC390726/