Tag

Part:BBa_K4673028:Design

Designed by: Chieh Yu Lee   Group: iGEM23_Taipei-KCISLK-V1   (2023-10-02)
Revision as of 15:43, 9 October 2023 by KCLK 23T (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


The his-tag can be detected by antibodies


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

His tag is located right after the Lumbrokinase gene and inserted before the T7 terminator gene on the pET-22b-LK vector.


Source

GSL Biotech LLC. (2023). pET-22b(+) Sequence and Map. SnapGene. https://www.snapgene.com/plasmids/pet_and_duet_vectors_(novagen)/pET-22b(%2B)

References

Morgenthal, J. B., Stephenson, J. R., Friesen, J. A., & Jones, M. A. (2006). An alternative to the traditional western blot for recombinant his-tagged proteins. American Journal of Biochemistry and Biotechnology, 2(4), 146–147. https://doi.org/10.3844/ajbbsp.2006.146.147