Coding

Part:BBa_K4891024

Designed by: Kexin Fei   Group: iGEM23_Guangzhou-MedX   (2023-09-19)
Revision as of 15:16, 9 October 2023 by KexinFei (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


talB

Phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P) could be stereospecifically condensed into 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) by tktA/talB gene encoding DAHP synthase.

Usage and Biology

We engineered the shikimate pathway of E. coli MG1655 to efficiently accumulate SA. We knocked out ptsG, ldhA, adhE, poxB, and pta genes to achieve the production of precursor substance PEP. To increase intracellular E4P content, we overexpressed tktA and talB genes. To enhance product accumulation, we overexpressed aroG, aroB, aroD, and aroE genes, while knocking down aroK and aroL genes to cut off the metabolic flux, thus accomplishing the accumulation of shikimic acid. Besides, a non-phosphorylated pathway, that is, glk and glf genes (mainly by glk-glf integration into the ptsG locus) is introduced to enhance glucose utilization. To achieve the goal above-mentioned, we totally constructed 26 parts this year. See table below:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 861
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None