Coding

Part:BBa_K4724015

Designed by: Qingqing Zheng   Group: iGEM23_NJTech-China-A   (2023-10-09)
Revision as of 15:07, 9 October 2023 by Registry (Talk | contribs)

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IsPETaseA240_S242del-6xHis Tag

We chose this mutation because asparagine is a polar amino acid, and deleting the polar amino acid weakens the polarity of the protein and promotes better binding of the protein to the hydrophobic surface of PET.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 637
    Illegal PstI site found at 56
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 787
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal AgeI site found at 546
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None