Regulatory

Part:BBa_K203107:Design

Designed by: Corinna Hiller and Hannah Uckelmann   Group: iGEM09_Heidelberg   (2009-10-15)
Revision as of 10:44, 19 October 2009 by Chiller (Talk | contribs) (→‎References)

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c-Jun promoter (growth factor regulated)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 398
    Illegal BamHI site found at 392
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 110


Design Notes

  • The plasmid with the promoter inserts was ordered from Addgene, Cambridge (USA):Plasmid 11979, pJC6-GL3
  • We used the following primers with BBb_standard for the amplification and isolation of the c-Jun promoter:
    • primer forward: 5'-GATCGAATTCGCGGCCGCACTAGTGCATGCCAATTCGAGCTCGG-3'
    • primer reverse: 5'-GATCCTGCAGCGGCCGCGCTAGCATCCAGATCTGGATCCAAGCAG-3'

Source

Addgene, Cambridge (USA):Plasmid 11979, pJC6-GL3

References

Clarke, N. et al. Epidermal Growth Factor Induction of the c-jun Promoter by a Rac Pathway. Mol Cell Biol 18, 1065–1073 (1998).