Regulatory

Part:BBa_K203107:Design

Designed by: Corinna Hiller and Hannah Uckelmann   Group: iGEM09_Heidelberg   (2009-10-15)
Revision as of 10:43, 19 October 2009 by Chiller (Talk | contribs) (→‎Source)

c-Jun promoter (growth factor regulated)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 398
    Illegal BamHI site found at 392
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 110


Design Notes

  • The plasmid with the promoter inserts was ordered from Addgene, Cambridge (USA):Plasmid 11979, pJC6-GL3
  • We used the following primers with BBb_standard for the amplification and isolation of the c-Jun promoter:
    • primer forward: 5'-GATCGAATTCGCGGCCGCACTAGTGCATGCCAATTCGAGCTCGG-3'
    • primer reverse: 5'-GATCCTGCAGCGGCCGCGCTAGCATCCAGATCTGGATCCAAGCAG-3'

Source

Addgene, Cambridge (USA):Plasmid 11979, pJC6-GL3

References