Coding

Part:BBa_K4724006

Designed by: Qingqing zheng   Group: iGEM23_NJTech-China-A   (2023-10-06)
Revision as of 14:11, 9 October 2023 by Moxi977 (Talk | contribs)


IsPETaseS93_I94insE-6xHis Tag

We inserted glutamate E between amino acids 93 and 94 of IsPETase.We chose this site because that the active pocket amino acids are changed, altering the tertiary structure of the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 793
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal AgeI site found at 549
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None