Coding

Part:BBa_K4724002

Designed by: Qingqing Zheng   Group: iGEM23_NJTech-China-A   (2023-10-06)
Revision as of 13:38, 9 October 2023 by Moxi977 (Talk | contribs)

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IsPETase

IsPETase is a polyethylene terephthalate degrading enzyme first identified in Ideonella sakaiensis. IsPETase can decompose PET and release soluble products such as MHET, and TPA. IsPETase's ability to degrade PET is limited by its low stability. The crystallinity of the substrate also affects the function of IsPETase in degrading plastics, and IsPETase degrades less effectively in PET samples with higher crystallinity.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal AgeI site found at 546
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None