Part:BBa_K4724084
ompA-LSPETase
The intracellular reducing environment is not conducive to the formation of protein disulfide bonds, and proteins with high levels of expression are prone to aggregation to form inclusion bodies. However, the oxidative environment in the periplasmic space of E. coli is conducive to the formation and stable existence of disulfide bonds. So guiding LSPETase into periplasmic space for expression is a good way to achieve soluble expression. Outer membrane protein A (OmpA) is an N-terminal signaling peptide that is a small molecule protein with a length of 20-30 amino acids and can transfer the produced protein to the periplasmic space. We connect signal peptides with LSPET target fragments to promote soluble expression of LSPETase by helping proteins fold correctly.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 895
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 151
Illegal AgeI site found at 238 - 1000COMPATIBLE WITH RFC[1000]
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