Part:BBa_K4765129

stem-loop test

Introduction

This compoiste part is a stem-loop-deleted version of BBa_K4765120, which includes StayGold and mScarlet. Its red-green fluorescence intensity ratio can be compared with BBa_K4765120 to assess the stem-loop's ability to prevent mRNA degradation.

Usage and Biology

We use this composite part to test the following stem-loops' ability to prevent mRNA degradation.

nsl:

5-AAACACCCACCACAAUUUCCACCGUUU UUUGU-3

liu2023:

 5-AAACACCCACCACAAUUUCCACCGUUU CCCGACGCUUCGGCGUCGGG UUUGU-3

new2:

 5-AAACACCCACCACAAUUUCCACCGUUU CCCCGUCGGCUGCU UUUGU-3

new6:

5-AAACACCCACCACAAUUUCCACCGUUU AGACGCUCGGCGUCCU UUUGU-3

new10:

 5-AAACACCCACCACAAUUUCCACCGUUU ACUGGGGGGAUCGAGGUCUUU UUUGU-3

bad2:

5-AAACACCCACCACAAUUUCCACCGUUU GCCGAUCGGGU UUUGU-3

bad6:

 5-AAACACCCACCACAAUUUCCACCGUUU AGACGCUCGGCGUCCU UUUGU-3

bad10:

5-AAACACCCACCACAAUUUCCACCGUUU GGCGGCGCUACAGCGUCGU UUUGU-3

Characterization

Sequencing map

Figure1 Sequencing map of stem-loop test Sanger sequencing verified we have removed the stem-loop before ribozyme sequence.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 1389
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 700
  Illegal BsaI.rc site found at 720