Coding

Part:BBa_K4623003:Design

Designed by: Ruixiao Tan   Group: iGEM23_BNU-China   (2023-10-09)
Revision as of 12:01, 9 October 2023 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


TrwC, a protein linker to covalently link ssDNA 5' end


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 286
    Illegal PstI site found at 604
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 286
    Illegal PstI site found at 604
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 286
    Illegal PstI site found at 604
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 286
    Illegal PstI site found at 604
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 409
    Illegal SapI.rc site found at 124


Design Notes

The sequences were codon-optimized for E.coli and a stop codon was added at the C-terminus


Source

Amino acid series were obtained from ref and transformed into corresponding DNA sequences, and codon optimization was performed against E.coli.

References