Coding

Part:BBa_K4765015

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-07-13)
Revision as of 07:12, 9 October 2023 by Siliang Zhan (Talk | contribs) (→‎Characterization)


AnAFP contributed by Fudan iGEM 2023

Introduction

Ammopiptanthus nanus antifreeze protein (AnAFP) is an antifreeze protein derived from Ammopiptanthus nanus. It protects the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions[1].

Usage and Biology

We heterologously expressed codon-optimized AnAFP in E.coli, endowing it with antifreeze capability.

Characterization

Sequencing map

contributed by Fudan iGEM 2023
Figure1 Sequencing map of AnAFP

Sequencing was performed using the two primers：T7: 5-TAATACGACTCACTATAGG-3 and Kan-F: 5-ATTCTCACCGGATTCAGT-3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 451


References

  1. ↑ Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang, S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in Escherichia coli and tobacco. Gene, 539(1), 132-140.https://doi.org/10.1016/j.gene.2014.01.013
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//cds
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protein