Tag

Part:BBa_K4613016:Design

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-09)
Revision as of 06:51, 9 October 2023 by YuchenZhou (Talk | contribs)

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SUMO-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 101
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

By construction of a fusion protein, M-CPA, with SUMO-tag, we increased the solube expression rate of M-CPA in Escherichia coli.


Source

Drosophila melanogaster

References

Panavas T, Sanders C, Butt T R.SUMO fusion technology for enhanced protein production in prokaryotic and eukaryotic expression systems[J].Methods Mol Biol,2009, 497: 303-17.