Part:BBa_K4645001
NeuA: scFv ( single-chain fragment variable）that inhibits the immune response to the feline
Velocimmune mice were immunized with recombinant dimeric Fel D1 and produced polyclonal antibodies. Among the resulting polyclonal antibodies, a monoclonal antibody named REGN1908 was found to be the most effective in blocking the immune response to the recombinant dimeric Fel D1. Through a literature review, we discovered both light chain and heavy chain genes of REGN1908. By fusing the C-terminal of the light chain with the N-terminal of the heavy chain using a rigid linker (GGGGS)3, an scFv was obtained. An OmpA signal peptide was fused to the N terminal of the scFv to secrete it into the periplasmic space, where it can more effectively neutralize the antigen.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 315
Illegal SpeI site found at 75 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 315
Illegal SpeI site found at 75 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 315
Illegal XhoI site found at 693 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 315
Illegal SpeI site found at 75 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 315
Illegal SpeI site found at 75 - 1000COMPATIBLE WITH RFC[1000]
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