Composite

Part:BBa_K4585016

Designed by: Siyao Ma   Group: iGEM23_CSU-CHINA   (2023-10-08)
Revision as of 03:57, 9 October 2023 by Registry (Talk | contribs)

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pcDNA3.1(+)-GnRH-C linearized vector

The purpose of constructing this linear vector was to perform homologous recombination with the GnRH-C homologous recombination fragment (BBa_K4585000) to obtain the pcDNA3.1(+)-GnRH-C plasmid (BBa_K45850011).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 249
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 895
    Illegal SpeI site found at 249
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
    Illegal XhoI site found at 901
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 249
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 249
    Illegal NgoMIV site found at 1341
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 882


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Categories
Parameters
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