Composite
Part:BBa_K4585016
Designed by: Siyao Ma Group: iGEM23_CSU-CHINA (2023-10-08)
pcDNA3.1(+)-GnRH-C linearized vector
The purpose of constructing this linear vector was to perform homologous recombination with the GnRH-C homologous recombination fragment (BBa_K4585000) to obtain the pcDNA3.1(+)-GnRH-C plasmid (BBa_K45850011).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 249
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 895
Illegal SpeI site found at 249 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 12
Illegal XhoI site found at 901 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 249
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 249
Illegal NgoMIV site found at 1341 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 882
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Categories
Parameters
None |