Coding

Part:BBa_K4613013

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-08)
Revision as of 02:51, 9 October 2023 by Registry (Talk | contribs)

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T3-M-CPA

T3(BBa_K4613011) and C3(BBa_K4613012) could form protein complexes by elastin-like polypeptides(ELPs) monomers containing SpyTags and SpyCatchers. Different functional proteins can be incorporated into the polymeric scaffolds in a flexible manner due to its programmability. In this part, NAU-CHINA 2023 incorporated Mature Carboxypeptidase A(M-CPA), which is capable of hydrolyzing OTA into the non-toxic product ochratoxin α and L-α-phenylalanine(Phe) in a high degration rate. We fused M-CPA into T3 to immobilize the enzyme and increase the stability and sustainable production of M-CPA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 995
    Illegal BamHI site found at 940
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1105
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 8


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Categories
Parameters
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