Coding

Part:BBa_K4613011

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-08)
Revision as of 02:19, 9 October 2023 by YuchenZhou (Talk | contribs)


T3

This part codes for hydrophilic elastin-like polypeptides (ELPs) fused with three SpyTag sequences. With C3 , they can form polymeric scaffolds via covalent bonding between SpyTag and SpyCatcher. If you want to learn about the detailed introduction of ELPs, you can click the link below. https://parts.igem.org/Part:BBa_K4613010

Reference

  1. Dai Z, Yang X, Wu F, et al.Living fabrication of functional semi-interpenetrating polymeric materials[J].Nature Communications,2021, 12 (1): 3422.
  2. Zakeri B, Fierer J O, Celik E, et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proc Natl Acad Sci U S A,2012, 109 (12): E690-7.
  3. Reddington S C, Howarth M.Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher[J].Curr Opin Chem Biol,2015, 29: 94-9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None