Composite
Part:BBa_K4579055:Design
Designed by: Vibhav Iyengar Group: iGEM23_Austin-UTexas (2023-10-08)
VanR regulated Mcc04 expression plasmid
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 180
Illegal PstI site found at 358 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 51
Illegal SpeI site found at 180
Illegal PstI site found at 358 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1078
Illegal BamHI site found at 37
Illegal BamHI site found at 75
Illegal BamHI site found at 1433
Illegal XhoI site found at 1468 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 180
Illegal PstI site found at 358 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 180
Illegal PstI site found at 358
Illegal AgeI site found at 291 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
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Source
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