Composite

Part:BBa_K5023013

Designed by: Lucas Daniel Ovelar Vargas   Group: iGEM23_UNILA-LatAm   (2023-10-08)
Revision as of 21:17, 8 October 2023 by Registry (Talk | contribs)

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PAR_Ble_SP7_FAST-PETase_mCherry_Ter

With the PAR promoter, the bleomycin/zeocin resistance sequence, signal peptide, the Fast-PETase, gene reporter mCherry, and the terminator. Designed to express and secrete Fast-PETase with verification by mCherry reporter in Chlamydomonas reinhardtii, utilizing pJP32 backbone.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 698
    Illegal PstI site found at 2111
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal NheI site found at 1211
    Illegal PstI site found at 698
    Illegal PstI site found at 2111
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1616
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 698
    Illegal PstI site found at 2111
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 698
    Illegal PstI site found at 2111
    Illegal NgoMIV site found at 1079
    Illegal NgoMIV site found at 1140
    Illegal NgoMIV site found at 1420
    Illegal NgoMIV site found at 3174
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None