Composite

Part:BBa_K4604024:Design

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-08)
Revision as of 20:13, 8 October 2023 by Registry (Talk | contribs)

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piG_23 (tetR_mazF)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 701
    Illegal XbaI site found at 616
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 701
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 701
    Illegal BglII site found at 710
    Illegal BamHI site found at 1105
    Illegal XhoI site found at 1114
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 701
    Illegal XbaI site found at 616
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 701
    Illegal XbaI site found at 616
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This was set up to test the toxicity of MazF without the regulation of the AdoCbl riboswitch used in BBa_K4604018.


Source

Parts of BBa_K4604018 were inserted into a backbone from our PI with Gibson Assembly.

References