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Composite
Part:BBa_K4604026:Design
Designed by: Hannah Swientek Group: iGEM23_Freiburg (2023-10-08)
piG_K12BSa (LacIprom_riboK12_lacI_trcProm_sfGFP)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 215
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
If we had placed the sfGFP/marker protein directly after the riboswitch, its negative regulation in the presence of AdoCbl would have resulted in a decrease in fluorescence. In this BioBrick the riboswitch is placed in front of a repressor gene (lacI) which inturn suppresses sfGFP expression. If the riboswitch is triggered by binding of AdoCbl, the repressor expression is stopped leading to a detectable fluorescent signal.
Source
Cloned with Gibson Assembly.