Composite

Part:BBa_K4604026:Design

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-08)
Revision as of 19:29, 8 October 2023 by Registry (Talk | contribs)

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piG_K12BSa (LacIprom_riboK12_lacI_trcProm_sfGFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 215
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If we had placed the sfGFP/marker protein directly after the riboswitch, its negative regulation in the presence of AdoCbl would have resulted in a decrease in fluorescence. In this BioBrick the riboswitch is placed in front of a repressor gene (lacI) which inturn suppresses sfGFP expression. If the riboswitch is triggered by binding of AdoCbl, the repressor expression is stopped leading to a detectable fluorescent signal.


Source

Cloned with Gibson Assembly.

References