Composite

Part:BBa_K4880007:Design

Designed by: Xinran Xia   Group: iGEM23_BASIS-China   (2023-08-26)
Revision as of 17:41, 8 October 2023 by Vivixia (Talk | contribs) (→‎Design Notes)

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Ptrc-theo-PtPS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1322
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 55
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1989


Design Notes

In our design, the expression of pinene synthase is controlled by IPTG and theophylline. Transcription is regulated by IPTG and translation initiates only when there is theophylline present.

Source

This composite part combines BBa_K3930029 and BBa_K4880003.

References