Coding
Part:BBa_K4632002:Design
Designed by: Haolong Lai Group: iGEM23_SCAU-China (2023-10-08)
Cry3A-like toxin
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Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1813
Illegal PstI site found at 295
Illegal PstI site found at 304
Illegal PstI site found at 1456 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1813
Illegal PstI site found at 295
Illegal PstI site found at 304
Illegal PstI site found at 1456 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1813
Illegal BglII site found at 533 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1813
Illegal PstI site found at 295
Illegal PstI site found at 304
Illegal PstI site found at 1456 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1813
Illegal PstI site found at 295
Illegal PstI site found at 304
Illegal PstI site found at 1456
Illegal AgeI site found at 1495 - 1000COMPATIBLE WITH RFC[1000]
===Design Notes===
666
References
[1] Lee A. Bulla, Jr.Mehmet Candas Formicidae (ant) control using Bacillus thuringiensis toxin US 6,551,800B1[P]. 2003-04-22.