Part:BBa_K4722008
3-Succinoyl-pyridine
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
3-Succinoyl-pyridine (SP) is a precursor compound with pivotal significance in the synthesis of depressant substances[1]. The biosynthesis of SP is intricately linked to the bioconversion of tobacco waste and is executed within the entirety of the cellular structure of the genetically engineered Pseudomonas strain S16dspm. SP represents a derivative of nicotine, characterized by its molecular simplicity as a pyridine compound. Remarkably, this compound possesses the capacity for transformation into a mammalian antihypertensive agent, namely ω-isopropyl-(propionyl)-L-proline[2].
Design Consideration
The genetic construct was ligated into a pET28a plasmid vector and subsequently introduced into Escherichia coli strain BL21 (DE3). NicX was genetically connected with SP to enable its direct translation onto the surface of BL21. This innovation eliminated the need for protein purification steps, allowing for the direct utilization of E. coli as a host for enzymes in various applications.
Enzyme Activity
TBD
References
- ↑ Wang, S. N., Liu, Z., Tang, H. Z., Meng, J., & Xu, P. (2007). Characterization of environmentally friendly nicotine degradation by Pseudomonas putida biotype A strain S16. Microbiology, 153(5), 1556-1565. https://doi.org/10.1099/mic.0.2006/005223-0
- ↑ Wang, W., Xu, P., & Tang, H. (2015). Sustainable production of valuable compound 3-succinoyl-pyridine by genetically engineering Pseudomonas putida using the tobacco waste. Scientific Reports, 5(1), 16411. https://doi.org/10.1038/srep16411
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