Composite

Part:BBa_K4880016:Design

Designed by: Xinran Xia   Group: iGEM23_BASIS-China   (2023-09-08)
Revision as of 17:31, 8 October 2023 by Vivixia (Talk | contribs) (→‎Design Notes)

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Ptrc-theo-αPS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1361
    Illegal XhoI site found at 1441
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our design, the expression of α-pinene synthase is controlled by IPTG and theophylline. Transcription is regulated by IPTG and translation initiates only when there is theophylline present.

Source

This composite part combines BBa_K3930029 and BBa_K517002.

References