Composite

Part:BBa_K4880005:Design

Designed by: Xinran Xia   Group: iGEM23_BASIS-China   (2023-08-26)
Revision as of 17:30, 8 October 2023 by Vivixia (Talk | contribs) (→‎Design Notes)

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SaSS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1023
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1023
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1023
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1023
    Illegal AgeI site found at 55
    Illegal AgeI site found at 984
    Illegal AgeI site found at 1623
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1418


Design Notes

In our design, the expression of santalene synthase is controlled by IPTG and theophylline. Transcription is regulated by IPTG and translation initiates only when there is theophylline present.

Source

This composite part combines BBa_K3930029 and BBa_K4880001.

References