Composite

Part:BBa_K4743002

Designed by: Jen Hsien, Liu   Group: iGEM23_PTSH-Taiwan   (2023-09-16)
Revision as of 15:50, 8 October 2023 by JenL (Talk | contribs)


NadA+ADH1 terminator with adapter

NadA gene codes for quinolinate synthase, which turns iminoaspartate into quinolate. The whole pathway is shown below:


NAD biosynthesis, iminoaspartate => quinolinate => NAD

Length checking of nadA

We use electrophoresis to check the length of the fragment

Figure 1. The length of NadA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1284
    Illegal SpeI site found at 1089
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1284
    Illegal SpeI site found at 1089
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1284
    Illegal BamHI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1284
    Illegal SpeI site found at 1089
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1284
    Illegal SpeI site found at 1089
    Illegal AgeI site found at 1297
  • 1000
    COMPATIBLE WITH RFC[1000]


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