Composite

Part:BBa_K4800042

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-08)
Revision as of 15:11, 8 October 2023 by Registry (Talk | contribs)

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pTrc99a-Spo3471

Aspartate transaminase from Ruegeria pomeroyi. A pyridoxal-phosphate protein. Also acts on L-tyrosine, L-phenylalanine and L-tryptophan. Aspartate transaminase activity can be formed from the aromatic-amino-acid transaminase (EC 2.6.1.57) of Escherichia coli by controlled proteolysis , some EC 2.6.1.57 activity can be found in this enzyme from other sources ; indeed the enzymes are identical in Trichomonas vaginalis .

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4051
    Illegal PstI site found at 2666
    Illegal PstI site found at 4063
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2666
    Illegal PstI site found at 4063
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4045
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4051
    Illegal PstI site found at 2666
    Illegal PstI site found at 4063
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4051
    Illegal PstI site found at 2666
    Illegal PstI site found at 4063
    Illegal NgoMIV site found at 3326
    Illegal AgeI site found at 3313
    Illegal AgeI site found at 3437
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4247
    Illegal BsaI site found at 5315
    Illegal SapI.rc site found at 767


[edit]
Categories
Parameters
None