![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4800042
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-08)
pTrc99a-Spo3471
Aspartate transaminase from Ruegeria pomeroyi. A pyridoxal-phosphate protein. Also acts on L-tyrosine, L-phenylalanine and L-tryptophan. Aspartate transaminase activity can be formed from the aromatic-amino-acid transaminase (EC 2.6.1.57) of Escherichia coli by controlled proteolysis , some EC 2.6.1.57 activity can be found in this enzyme from other sources ; indeed the enzymes are identical in Trichomonas vaginalis .
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4051
Illegal PstI site found at 2666
Illegal PstI site found at 4063 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2666
Illegal PstI site found at 4063 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4045
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 4051
Illegal PstI site found at 2666
Illegal PstI site found at 4063 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4051
Illegal PstI site found at 2666
Illegal PstI site found at 4063
Illegal NgoMIV site found at 3326
Illegal AgeI site found at 3313
Illegal AgeI site found at 3437 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4247
Illegal BsaI site found at 5315
Illegal SapI.rc site found at 767
[edit]
Categories
Parameters
None |