Plasmid_Backbone

Part:BBa_K4769000:Design

Designed by: 2023 Cambridge iGEM   Group: iGEM23_Cambridge   (2023-10-08)
Revision as of 15:02, 8 October 2023 by Rosaline (Talk | contribs)

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Design Notes

pMiniMAD contains an internal BsaI cut site within the ampicillin resistance gene – this can be avoided when using BsaI by ensuring minimal homology of the internal 4 bp overhang with other designed overhangs and performing an additional final ligation step following Golden Gate assembly.

Alternatively, the BsaI recognition sequence could be removed by point mutation to a synonymous codon, by site-directed mutagenesis or using an additional internal set of primers.