Plasmid

Part:BBa_K4665171:Design

Designed by: Floor Vervuren   Group: iGEM23_MSP-Maastricht   (2023-10-08)
Revision as of 12:54, 8 October 2023 by FloorVervuren (Talk | contribs) (→‎Design Notes)

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HP17_KO7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 5958
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This plasmid can be used in combination with the

Source

The helper plasmid was constructed by Praetorius et al., (2017) by cloning the coding parts of helper phage M13KO7 into a pSC101 plasmid backbone carrying a kanamycin resistance gene.

References

Praetorius, F., Kick, B., Behler, K. et al. (2017). Biotechnological mass production of DNA origami.Nature 552, 84–87. https://doi.org/10.1038/nature24650

https://www.addgene.org/120346/