Composite

Part:BBa_K4604022:Design

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-08)
Revision as of 12:35, 8 October 2023 by Registry (Talk | contribs)

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piG_10b (tetR_bluB_T7RBS_riboK12_mazF)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1603
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2252


Design Notes

Since the inherent RBS of the riboswitch might be detremential for the correct folding of said, we decided to add an additional (supposedly stronger) RBS right after the riboswitch, in front of the desired gene.


Source

Modified BBa_K4604021 with Gibson assembly. The tet promoter was taken from iGEM Freiburg 2022 (BBa_K4229059).

References