Composite

Part:BBa_K4604022

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-08)
Revision as of 12:35, 8 October 2023 by Registry (Talk | contribs)

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piG_10b (tetR_bluB_T7RBS_riboK12_mazF)

Plasmid piG_10 is made up of the tet promoter/repressor, bluB, a riboswitch for AdoCbl, the tet RBS, mazF (toxin) and the rrnB terminator. The backbone we utilized was pGGAselect, a plasmid commonly used with golden gate cloning that has a chloramphenicol resistance. This BioBrick is BBa_K4604021 with an additional RBS.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1603
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2252


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