Composite

Part:BBa_K200022

Designed by: Royah Vaezi   Group: iGEM09_Imperial College London   (2009-10-15)
Revision as of 22:19, 18 October 2009 by Droche (Talk | contribs)

Heat-inducible system with GFP reporter

Ligation of heat repressible regulation system to GFP to assay its activity.

This part comprises the heat sensitive Lamda cI Repressor designed by Harvard iGEM 2008 (BBa_K098995) with a standard GFP reporter output (BBa_I13504). This part was used as a test construct, to check the functionality of the part.

We have shown (BBa_K098995) to be functional and have improved its characterisation through successful testing with this construct containing a GFP reporter. By measuring GFP flourescence at 28 degrees, 42 degrees and with a temperature shift from 28 to 42 degrees, the part showed functionality.


Usage and Biology

The Imperial iGEM 2009 Team successfully used this construct to show the functionality of this composite part. By measuring the increase in GFP flourescence over a temperature shift, the part was shown to be working successfully. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1599


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Categories
Parameters
None