Coding

Part:BBa_K4623004:Design

Designed by: Yixin Huang   Group: iGEM23_BNU-China   (2023-10-08)
Revision as of 11:07, 8 October 2023 by Registry (Talk | contribs)

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Basic Silinker (TrxA-His-thrombin-mSA-SBP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 445
    Illegal AgeI site found at 505
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is appended with an His tag, allowing for purification using a nickel column.


Source

It is a new fusion protein we have designed. We combined the sequence of Trx-His-thrombin-mSA-SBP.

References