Coding

Part:BBa_K4623004

Designed by: Yixin Huang   Group: iGEM23_BNU-China   (2023-10-08)
Revision as of 11:07, 8 October 2023 by Registry (Talk | contribs)

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Basic Silinker (TrxA-His-thrombin-mSA-SBP)

Basic Silinker (BS) is a novel recombinant protein that can efficiently attach to the surface of silicon dioxide. The sequence in FASTA format is appended with an HIS tag, allowing for purification using a nickel column. Upstream of the sequence, a trxA (part number)fusion tag is added to aid in protein folding and reduce the formation of inclusion bodies in bacterial cells. After protein expression, cleavage by thrombin (part number) exposes the mSA (Part number) site for binding with a biotinylated functional protein. The SBP (part number) sequence can bind to the surface of silicon dioxide, enabling the modification of functional proteins onto the surface.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 445
    Illegal AgeI site found at 505
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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