Translational_Unit

Part:BBa_K4879011:Design

Designed by: Apoorva Gopal   Group: iGEM23_IISER-Pune-India   (2023-10-08)
Revision as of 10:33, 8 October 2023 by Registry (Talk | contribs)

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ScRAD52 Expression Construct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1780
    Illegal suffix found in sequence at 2050
    Illegal EcoRI site found at 2044
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1780
    Illegal EcoRI site found at 2044
    Illegal SpeI site found at 192
    Illegal SpeI site found at 2051
    Illegal PstI site found at 157
    Illegal PstI site found at 2065
    Illegal NotI site found at 1786
    Illegal NotI site found at 2058
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1780
    Illegal EcoRI site found at 2044
    Illegal BglII site found at 766
    Illegal BglII site found at 896
    Illegal XhoI site found at 1586
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1780
    Illegal suffix found in sequence at 2051
    Illegal EcoRI site found at 2044
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1780
    Illegal EcoRI site found at 2044
    Illegal XbaI site found at 1795
    Illegal SpeI site found at 192
    Illegal SpeI site found at 2051
    Illegal PstI site found at 157
    Illegal PstI site found at 2065
    Illegal NgoMIV site found at 1570
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References