Part:BBa_K4897000

BS DNA-50 (BS DNA 1.0) using in L-RCA for detecting P. acne

What is it?

BS DNA-50 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. The binding region is the key element of binding the P. acne 16s rRNA gene [1]. The DNA ligase will perform the ligation of the single-strand DNA meanwhile the phi29 will generate double-stranded DNA through amplification primers

Usage and Biology

Fig. 1. The process of BS DNA binding to P. acne DNA.

Overall, theoretically, through ligation and rolling circle amplification (L-RCA), BS DNA-50 should have high selectivity for detecting the 16S rRNA gene of P. acne.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 27
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 27
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 27
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 27
  
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

[1]Nakamura, M., Kametani, I., Higaki, S., & Yamagishi, T. (2003). Identification of Propionibacterium acnes by polymerase chain reaction for amplification of 16S ribosomal RNA and lipase genes. Anaerobe, 9(1), 5–10. https://doi.org/10.1016/s1075-9964(03)00061-1.