Part:BBa_K4665170:Design

Octahedron DNA-origami (3024bp long; 42bp edges)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 919
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 919
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 738
  Illegal BglII site found at 1310
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 919
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 919
  Illegal NgoMIV site found at 132
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

After extracting the sequence from the plasmid, T7 exonuclease digestion will need to be performed in order to get ssDNA needed for DNA origami.

Source

The plasmid with the 3024bp sequence was obtained from addgene. The original plasmid was constructed by Nafisi et al., (2018). They converted a pUC18 vector into a pagemid for custom ssDNA production by adding four components: 1) a full-length M13 origin for ssDNA initiation. 2) Kpnl and BamHl restriction sites for insert cloning. 3) M13 PS for phage particle export. 4) modified M13 origin to serve as the ssDNA synthesis terminator.

References

Nafisi, P. M., Aksel, T., Douglas, S. M. (2018) Construction of a novel phagemid to produce custom DNA origami scaffolds, Synthetic Biology, Volume 3, Issue 1, https://doi.org/10.1093/synbio/ysy015