Composite

Part:BBa_K4767016:Design

Designed by: Fangjun Liu   Group: iGEM23_CUG-China   (2023-10-04)
Revision as of 09:13, 8 October 2023 by Airplan mod (Talk | contribs) (→‎Design Notes)

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RBS-TT-Pars-RBS-arsR-luxR(△2-162)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 427
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a combination of a strong RBS, a double terminator, followed by arsenic sensing and enhancer: ars promotor, RBS, arsR and luxR(△2-162).

Source

This part is from Escherichia coli and Vibrio fischeri,assembled by BBa_K4767001, BBa_J34801 , BBa_J15101 , BBa_B0015 and BBa_K4767000.

References

Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4.

Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.