Composite

Part:BBa_K200019

Designed by: Royah Vaezi   Group: iGEM09_Imperial College London   (2009-10-15)
Revision as of 20:16, 18 October 2009 by Royah (Talk | contribs)

pLacI+RBS+RFP+TT+pCstA+RBS+GFP+TT

This is the testing construct used by Imperial College's 2009 iGEM team to assess the two inducible promoters pLacI and pCstA through the expression of reporter molecules RFP and GFP in [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project.

The subparts used to make this composite are BBa_J5526 and BBa_K00018.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 636
    Illegal AgeI site found at 748
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1733


[edit]
Categories
Parameters
None