Part:BBa_K200020

pCstA+RBS

This BioBrick comprises a ligation of the registry parts for the promoter PcstA (BBa_K118011) and the RBS (BBa_B0034).

Promoter for the cstA gene ligated to a RBS.

This is the promoter for the Eschaerichia coli JM109 cstA gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (cstA encodes the carbon starvation protein.)

Usage and Biology

Imperial iGEM 2009 used the promoter as part of the autoinduction module of the project. By using minimal media combined with a secondary carbon source and limited glucose supply, the team aimed to characterise a time delay during cell growth after which the promoter will be activated.
Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]