Composite

Part:BBa_K4767009

Designed by: Fangjun Liu   Group: iGEM23_CUG-China   (2023-10-04)
Revision as of 08:07, 8 October 2023 by Airplan mod (Talk | contribs)

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PluxⅠ-RBS-luxR(△2-162)-RBS-cymA-TT

Uses a factor PluxⅠ(BBa_R0062) ,strong RBS(BBa_J34801), luxR(△2-162)(BBa_K4767000), cymA(BBa_K2706008)and TT(BBa_B0015). This composite part is a BioBrick.

Usage and Biology

This is the response part of the luxR amplifier with the cymA gene as the reporter. In our design, if luxR(△2-162) was expressed as a result of responding, this part can be activated to express cymA and luxR(△2-162). CymA will increase the ability for the Shewanella oneidensis to transfer the electrons, and luxR(△2-162) will functions in a positive feedback loop as it can bind to the PluxⅠ promoter and activate its own transcription.

img-1172.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 685
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4.

[edit]
Categories
//classic/reporter
Parameters
biologyVibrio fischeri & S. oneidensis