Composite

Part:BBa_K4767004

Designed by: Fangjun Liu   Group: iGEM23_CUG-China   (2023-10-04)
Revision as of 07:27, 8 October 2023 by Airplan mod (Talk | contribs)


Pars-RBS-arsR-RBS-gfP-TT

Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor arsR(BBa_J15101), gfp coding region(BBa_E0040)and double terminator TT(BBa_B015). This part is an easy BioBrick.

Usage and Biology

This part is a basic biosensor for arsenic detection using gfp as the reporter gene. When arsenic is absent, the transcription regulator ArsR binds to the ArsR-binding site (ABS) within the ars promoter and blocks transcription. Once arsenic is present, it binds to ArsR and activate the transcription of the downstream genes. Since this ars System comes from Escherichia coli genome, to eliminate this impact, we used another model bacteria Shewanella oneidensis as the chassis cells to verify the function of this part. As the result shows below, with the arsenic concentration rises, the strain with the reporter produced higher fluorescence intensity.

img-1166.png
Fig 1. Fluorescence curve of the reporter strain with different arsenic concentrations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 270
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1160


[edit]
Categories
//classic/reporter
Parameters
biologyEscherichia coli