Composite

Part:BBa_K190036:Design

Designed by: Michael Verhoeven   Group: iGEM09_Groningen   (2009-09-07)
Revision as of 18:58, 18 October 2009 by PaulSchavemaker (Talk | contribs) (→‎Design Notes)

GVP with LacI regulated promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 542
    Illegal BamHI site found at 4347
    Illegal XhoI site found at 4035
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 993
    Illegal NgoMIV site found at 1395
    Illegal AgeI site found at 4314
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2805
    Illegal SapI.rc site found at 3961


Design Notes

Melbourne GVP cluster was used containing the 400bp repeat in gvpL.

Source

E.coli and Bacillus

References